In the present study, we evaluated the protective effects of amentoflavon. Amentoflavone(AMF) is a naturally occurring plant alkaloid found in a number of different plants, most notably Gingko biloba and St John's Wort. Studies have shown that this compound possesses a plethora of benefits ranging from improving overall health to combating disease. It also has demonstrated a number of benefits for exercise, leading it to garner attention from trainers, athletes and supplement manufacturers, particularly those in the pre workout and fat burner market.
Amentoflavone inhibits adipocyte differentiation
The accumulation of body fat greatly depends on adipocyte differentiation, also known as adipogenic differentiation or adipogenesis. Adipocyte differentiation is a complex process which is divided into four steps, including initial growth arrest, mitotic clonal expansion (MCE), early differentiation and terminal differentiation, namely the development of the mature adipocyte phenotype. Adipocyte differentiation is regulated by a series of signaling pathways triggered by an adipogenic stimulus.
Amentoflavone (AMF) for metabolic dysfunction induced by high fat (HF) diet was assessed and focused on the effect of AMT on adipogenesis differentiation during 3T3-L1 adipocyte differentiation.
3T3-L1 pre-adipocytes were subjected to adipogenic differentiation in the presence of 1-10 µg/ml AMF for 8 days. (A) After differentiation, lipid accumulation in adipocytes was examined by Oil Red O staining and observed under a microscope. (B) The oil droplets were then dissolved in isopropanol and the absorbance of the dyeetriglyceride complex was measured at 510 nm. The results are expressed as folds of DMI. (C)
It was examined the effects of AMF on 3T3-L1 adipocyte differentiation in vitro. AMF inhibited the accumulation of oil droplets in the differentiated adipocytes in a concentration-dependent manner, indicating that AMF suppressed 3T3-L1 adipocyte differentiation. Moreover, we evaluated the effect of AMF on 3T3-L1 adipocyte differentiation during different stages of differentiation. It was indicated that AMF exerted inhibitory effects on 3T3-L1 adipocyte differentiation at different stages of differentiation.
Amentoflavone attenuates metabolic dysfunction
Type 2 diabetes and metabolic syndrome are prevalent world-wide. These metabolic disorders are characterized by the disruption of glucose and lipid metabolism.
The inhibitory effects of AMF on adipocyte differentiation contribute to its protective effects against HF diet-induced metabolic dysfunction. Overall, the data in our study provide novel insight into the mechanisms responsible for the protective effects of AMF against HF diet-induced metabolic dysfunction and those for its inhibitory effect on adipocyte differentiation.
Schematic diagram of the molecular mechanisms underlying the amentoflavone (AMF) induced protective effects against adipogenic differentiation and metabolic dysfunction.
In conclusion, in the present study, we found that AMF attenuated metabolic dysfunction in HF diet-fed rats and exerted inhibitory effects on 3T3-L1 adipocyte differentiation. The data from the present study provide novel insight into the mechanisms underlying the protective effects of AMF against metabolic dysfunction and the inhibitory effects of AMF on adipocyte differentiation. And fat gain in the process of weight loss also has a good description.
Diabetes, often referred to by doctors as diabetes mellitus, des... More
According to research, myricetin stimulates glucose transport in... More
There are many studies at the molecular, biochemical, organism a... More
In vivo, betaine acts as a methyl donor for the conversion of h... More
Apigenin, an abundant dietary ﬂavonoid, is emerging as a potent... More
BVC synergised with thiazolidinediones, which are synthetic PPAR... More
Epicatechin is an antioxidant flavonoid, occurring especially in... More
Fatigue is a very common phenomenon. There are many reasons can ... More
There are some simple steps can be taken to prevent or slow bone... More
Cardiac hypertrophy is the myocardial response to various pathol... More
Obesity has become a global health issue. It is a medical condit... More
Echinacea is also called the magic herb that can bring health to... More
Aging is becoming an international challenge to healthcare syste... More
Asthma is a common long-term inflammatory disease of the airways... More
Studies have shown that this compound possesses a plethora of b... More
β-Glucan is an important functional ingredient having ... More
Taking fish oil is beneficial for the human body in va... More
Spermidine and spermine which belong to polyamines,can... More
Most of us know that copper is a mineral but what many... More